金银忍冬花粉离体萌发初探

A Preliminary Study of Pollen Germination in vitro of Lonicera maackii (Rupr.) Maxim.

摘要:

以金银忍冬[Lonicera maackii (Rupr.) Maxim.]为试材，研究了不同种类糖、硼酸、钙离子、pH值、光照等单因子对花粉萌发的影响。结果表明，培养基中分别含有25%的蔗糖、0.01%的硼离子、0.02%~0.05%的钙离子以及pH 6.7时较适合金银忍冬的花粉萌发，萌发率分别为85.63%、59.23%、78.12%和75.27％；培养基中不同种类的糖对花粉萌发的影响不同，以乳糖最适宜，其次是葡萄糖、蔗糖和果糖；在光照(0.5625 μmol m-2s-1)与黑暗下培养，金银忍冬花粉的萌发率没有明显的变化，但黑暗条件更利于花粉管的生长。金银忍冬花粉在含有25%蔗糖、0.01%硼离子、0.02%钙离子、pH为6.7的培养基上光照培养3 d，其花粉萌发率均达到80%以上，最高的萌发率可达88.1％。

Abstract:

The effects of sucrose, boric acid, calcium, pH, and illumination on the fresh pollen germination of Lonicera maackii (Rupr.) Maxim. were studied. The results shown that the pollen germination rate were 85.63%, 59.23%, and 78.12%, when medium supplied with 25% sucrose, 0.01% boric acid, 0.02%~0.05% Ca(NO3)2, respectively, and the germination rate got 75.27％ cultured under pH 6.7. The effects of carbohydrates on pollen germination rate in order were sucrose＞glucose＞lactose ＞fructose. There was not significant difference of pollen germination rate under illumination (0.5625 μmol m-2s-1) and dark, but the length of pollen tube was longer under the dark than under the illumination. The optimal medium for pollen germination consist of 25% sucrose, 0.01% boric acid, 0.02% Ca(NO3)2 at pH 6.7 and under illumination, in which the pollen germination rate reached 88.1％.