云南火焰兰种子无菌萌发和试管育苗

Seed Germination in vitro of Renanthera imschootiana Rolfe

摘要:

对云南火焰兰进行无菌播种技术试验。结果表明，适宜萌动培养基为1/4MS+椰子水 20％＋蛋白胨 1 g L-1＋蔗糖 10 g L-1，播种30 d时萌动率可达93.1%；适宜萌发培养基为VW+ 椰子水 10％，培养75 d时萌发率达36.6%。盐浓度较高的MS培养基不适合种子萌发和原球茎生长，低盐的KC培养基上种子能较好地萌发和成苗；添加椰子水可明显提高种子萌动率，并有利于原球茎的生长和分化。光培养和暗培养对种子萌动的影响差异不明显，但暗培养3周会对种子后期发育不利。添加150 g L-1的苹果匀浆有利于幼苗生长。试管苗移栽成活率在95%以上。

Abstract:

Asymbictic germination of an endangered orchid, Renanthera imschootiana Rolfe was achieved through sowing the seeds after pollinating for 145 d on different mediums axenically. In 30 d after sowing, the seed germination rate reached 93.1% on the 1/4MS medium supplemented with 20% （v/v） coconut milk（CM）, 1 g L-1 peptone and 10 g L-1 sucrose. After 75 d, on the VW medium with 10% CM, 36.6% of the seed formed plantlets. The content and concentration of multi-salt of basic medium has a positive (or great) effect on seed germination and plantlet growing. 1 g L-1 peptone increased seed germination rate, while 150 g L-1 apple homogenate can enhance plantlet growth. Seed germination rate was found no significant difference when seeds were sowed in light or dark, but 3-weeks culture in dark had a negtive effect on developing seedlings after seed germination. More than 95% seedlings were survival after transplanted to the g