Abstract:Twelve hybrid crosses among 3 cytoplasmic male sterile lines and 4 varieties of flue-cured tobacco popular in China were used to determine the heterosis in the following economic characters: yield, production value, average price, high class tobacco ratio, high and middle class tobacco ratio and tobacco class index. Results were as follows: (1) Heterosis from mid-parent was found in most crosses, and the yield and production value in 100.00% crosses were significantly different at 0.05 or 0.01 levels, so were the other economic characters in 12.50%-83.33% crosses; (2) Heterosis over higher-parent for yield and high class tobacco ratio was found in most crosses, and the heterosis of yield was in 75.00% and high class tobacco ratio in 66.67% crosses were significant at 0.05 or 0.01 levels, respectively. The others economic characters were in 8.33%-50.00% of crosses with significance at 0.05 or 0.01 levels respectively. (3) The cross of MsYZ203-6 × Honghuadajinyuan showed significant heterosis over higher-parent in all economic characters expressed above, and cross of MsYZ206-9 × Yunyan87 showed significant heterosis over higher-parent in all economic characters above except average price.