硫和pH对蛋白核小球藻光照产氢的影响

Effects of Sulfur and pH on Photohydrogen Production of the Green Alga Chlorella pyrenoidosa

摘要:

在有无硫及pH 5.0-8.0下对蛋白核小球藻(Chlorella pyrenoidosa)光照产氢的影响进行了研究。结果表明，在持续光照（165 μmol m-2s-1）条件下，从有硫培养液（TAP培养液）内叶绿素a含量、Fv/Fm值及ΦPSII值的变化表明蛋白核小球藻在pH 6.0-7.0时生长最佳，生长旺盛易形成暂时的无氧环境而利于藻产氢。最高的产氢速率和总产氢量出现在pH 7.0，分别是0.10 ml mg-1 chl h-1和1.39 ml。从无硫培养液（TAP-S培养液）内叶绿素a含量、Fv/Fm值及ΦPSII值的变化表明蛋白核小球藻生长明显受抑制，形成的无氧环境持久，故产氢持久，总产氢量比有硫培养液内高。蛋白核小球藻在pH 5.5培养液内的Fv/Fm值后期高于其他4种pH值的，表明潜在的PSII光化学效率高，在光照条件下产氢电子主要来源于PSII，故 pH 5.5的无硫培养液内藻的产氢速率和总产氢量最大，分别是0.58 ml mg-1 chl h-1和10.98 ml。说明pH为5.5的无硫培养液是蛋白核小球藻产氢的最佳条件。

Abstract:

The effects of sulfur and pH(5.0-8.0) on photohydrogen production was investigated in a unicellular green alga, Chlorella pyrenoidosa. Under continuous illumination of 165 μmol m-2s-1 conditions, in the TAP culture medium, changes of chlorophyll a content、Fv/Fm and ΦPSII indicated that growth of C. pyrenoidosa were very well at initialcultivation pH ranged from 6.0 to 7.0 and can form a temporary anoxic conditions in favor of H2 production. Maximum H2 production was obtained at initial cultivation pH 7.0 by C. pyrenoidosa. The maximum rate of H2 produced by C. pyrenoidosa was 0.10 ml mg-1 chl h-1 and the maximum total yield of H2 produced was 1.39 ml. In TAP-S culture medium, changes of chlorophyll a content、Fv/Fm and ΦPSII indicated that growth of C. pyrenoidosa were inhibited and can form a permanent anoxic conditions in favor of H2 production. The total yield of H2 production by C. pyrenoidosa in TAP-S culture was more than that in TAP culture. The Fv/Fm value of C. pyrenoidosa in pH 5.5 TAP-S culture was higher than the others culture. It indicated the residual PSII was more than the others. Under continuous illumination the electron of production H2 requirement mostly comes from PSII, so the maximum rate and total yield of H2 produced by C. pyrenoidosa appeared in pH 5.5 TAP-S cultures. The maximum rate of H2 produced by C. pyrenoidosa was 0.58 ml mg-1 chl h-1 and the total yield of H2 produced was 10.98 ml. The H2 production of C. pyrenoidosa was notable effected by sulfur and pH. In this test the best conditions for H2 production of C. pyrenoidosa was in pH 5.5 TAP-S cultures.