航天诱变百脉根结瘤突变体的筛选

Screening for Root Nodulation-related Mutants of Lotus japonicus Induced by Space Flight

摘要:

通过返回式卫星搭载，利用太空环境对百脉根（Lotus japonicus）MG-20种子进行诱变。从种植的三代植株中，筛选到多种共生固氮根瘤的突变体, 其中，不结根瘤突变体18个株系，表现为接种根瘤菌两周后无根瘤形成；结无效根瘤突变体9个株系，表现为根瘤数目少且分布不均匀，根瘤呈白色，有些为半透明；花叶形态异常突变体株1个系，表现为除根瘤数目少外，植株矮小、托叶消失、花形态异常；纤细突变体1个株系，表现为除根瘤数目少外，植株变小、茎细叶小。

Abstract:

Lotus japonicus MG-20 seeds were exposed to space environment by space flight using a recoverable satellite. After the selection from one to three generations, several mutants were obtained。The major mutants included non-nodule mutants (18 lines) which failed to nodulate after inoculated with rhizobia for two weeks, ineffective nodulation mutants (9 lines) which had less uneven and white or transparent nodules, an abnormal leaf and flower mutant which was dwarf without stipules, abnormal flowers and less nodules, and a slender mutant with a thin stem and smaller leaves.