花烛无菌苗液体增殖培养的影响因子

Auxiliary Bud Proliferation of Anthurium andraeanum Lind. in Liquid Culture

摘要:

以花烛 (Anthurium andraeanum Lind.) 品种Sonate无菌苗为材料, 研究了液体培养条件下不同植物生长调节剂、蔗糖浓度、培养方式等因子对腋芽增殖的影响, 并比较了Sonate、Valentino和Julanba3个品种间腋芽增殖的差异。结果表明: 在液体振荡培养条件下, 以Nitsch + BA 0.5 mg L^-1+ KT 1.0 mg L^-1+ 蔗糖30 g L^-1为Sonate适宜的增殖培养基; 在20 μmol m^-2s^-1弱光下培养25 d后转至40 μmol m^-2s^-1正常光照下培养, 对Sonate增殖效果较好,平均腋芽数可达11.1, 平均腋芽长为1.4 cm; 花烛腋芽增殖能力品种间存在差异,但3个品种均可在以上培养基和光照条件下快速增殖, 其中以Sonate和Valentino增殖较快。

Abstract:

The influences of different combinations of plant regulators, sucrose concentration and culture modes on proliferation rates and the quality of auxiliary buds were studied in Anthurium andraeanum cv. Sonate in liquid culture. Differences in the proliferation were compared among A. andraeanum cvs. Sonate, Valentino and Julanba. Nitsch basal medium supplemented with 0.5 mg L^-1 BA, 1.0 mg L^-1 KT and 30 g L^-1 sucrose was suitable for auxiliary bud proliferation of Sonate under liquid flask-shaking culture condition. Auxiliary bud proliferation of Sonate was promoted by the light of 20 μmol m^-2s^-1 of 25-day culture followed by 40 μmol m^-2s^-1, with the bud proliferation rate of 11.1 and the average bud length of 1.4 cm. The cultivars, Sonate, Valentino and Julanba, were well propagated under the above culture condition, especially Sonate and Valentino, but they showed differences in auxiliary bud emergence.