乙烯利调控甘蔗生长前期基因表达初报

Differential Gene Expression in Sugarcane Leaves Regulated by Ethephon During Early Growth Stage

摘要:

在甘蔗生长前期分别叶面喷施200 mg/L乙烯利和清水，利用cDNA-AFLP技术分析甘蔗在两种不同处理后体内基因表达差异情况，以期通过对差异表达基因进行序列分析和功能分析，探讨乙烯利调控甘蔗生长的分子机理。结果表明：经乙烯利处理后的甘蔗叶片，cDNA-AFLP产物多态性丰富，基因表达差异显著；部分差异片断序列与抗病、抗逆及促进光合作用等相关基因具有较高的同源性。

关键词:甘蔗;乙烯利;cDNA-AFLP;基因差异表达

Abstract:

A study was conducted to investigate differential gene expression in sugarcane (ROC16) leaves subjected to 200 mg/L ethephon. cDNA-AFLP amplification showed a high level of polymorphism，and gene expression was significantly different compared with control. Some sequences of different expression fragments were highly homologous to disease-resistant and photosynthesis-associated genes.

Key words:Sugarcane; Ethephon; cDNA-AFLP; Differential gene expression