基因枪法转化水稻E32后代非目标农艺性状变异的研究

Variation of Non-target Agronomic Traits in the Offspring of Transgenic Rice Line E32 by Particle Bombardment

摘要:

采用基因枪法将4个抗真菌性病害基因（RAC22、RCH10、β-1,3-Glu和B-Rip）导入超级稻（Oryza sativa L.）恢复系E32中，经选择获得10个T₄代转基因株系。分蘖盛期纹枯病抗性鉴定表明外源基因已在转基因株系中得到表达并获得抗性，这些转基因株系除了产生抗病性等目标性状的变异外，还发生生育期、植株形态、谷粒外观及产量性状等非目标农艺性状变异。对这些非目标性状进行遗传增益和稳定性分析表明，不同转化株系间或性状间的遗传增益均存在较大差异，其中遗传增益最高的性状是单株穗数、着粒密度和单株产量；各性状中以株高、穗长和谷粒外观性状的变异程度最低，单株穗数和千粒重的变异系数最大。通过选择可获得产量与品质性状均有提高的转基因水稻。

Abstract:

Four antifungal protein genes (RAC22、RCH10、β-1,3-Glu and B-Rip) were introduced into Indica hybrid rice (Oryza sativa L.) restorer line E32 by particle bombardment. Ten transgenic progenies of T₄ generation exhibited resistance to sheath blight after inoculated at the active tillering stage. In addition, the transgenic progenies had obvious variations in some non-target agronomic traits, such as growth duration, morphology, grain appearance and grain yield. The genetic gains were different among transgenic lines and the traits. Panicle, grain density and grain yield per plant had the highest genetic gains. The variation coefficients of plant height, panicle length and grain appearance were low, while those of panicle numbers per plant and 1000-grain weight reached high levels. The transgenetic rice cultivars with high yield and good quality could be developed.