长穗颈温敏核不育水稻穗颈节间长度及细胞学观察

Variations of Length and Cell Numbers of the Uppermost Internode of TGMS Rice with eui Gene

摘要:

以水稻培矮64S作对照，对隐性长穗颈温敏核不育水稻(Oryza sativa L.)长选3S幼穗各发育时期穗颈节间长度及细胞数量和长度进行比较分析。结果表明，长选3S在幼穗分化的二核期至始花期穗颈节间伸长速度最快，其节间伸长的长度是培矮64S的2.1倍；在花粉母细胞减数分裂期至二核期及始花当天至始花后第3天两个时段，穗颈节间伸长速度慢。穗颈节间的细胞个数和平均长度两者呈现相似的变化规律，但从花粉母细胞减数分裂期至始花期，长选3S穗颈节间薄壁细胞增加3 759个，细胞平均长度为72.9 μm，培矮64S增加3 134个，细胞平均长度为38 μm，长选3S穗颈节间的伸长主要是由幼穗分化的花粉母细胞减数分裂期至始花期细胞分裂和细胞伸长共同作用的结果，其中后者的作用更显著。

Abstract:

Changxuan 3S is a thermo-sensitive genetic male-sterile (TGMS) rice (Oryza sativa L.) with eui gene transferred from cultivar Pei’ai 64S. Variations in length and cell number of the uppermost internode were compared between the two cultivars during various stages of panicle initiation. The uppermost internode of Changxuan 3S elongated fastest during binucleate to anthesis stages, consequently it reached 2.1 times as long as that of Pei’ai 64S. Then it elongated slowly during pollen mother cell meiosis to binucleate stages and during 1 to 3 days after anthesis. During pollen mother cell meiosis to anthesis stages, parenchyma cells of the uppermost internodes of Changxua 3S and Pei’ai 64S increased by 3 759 and 3 134 in numbers, respectively, and with average cell lengths of 72.9 and 38 μm, respectively. The uppermost internode of Changxua 3S elongated due to cell division, especially cell elongation during pollen mother cell meiosis to anthesis stages.