蝴蝶兰叶片外植体褐变过程中PAL基因的表达变化

Phenylalanine Ammonia-Lyase (PAL) Gene Expression Correlated with Phalaenopsis sp. Leaf Explant Browning

摘要:

研究蝴蝶兰(Phalaenopsis. sp)叶片外植体褐变过程中PAL基因表达变化。结果发现在整个褐变过程中，外植体的PAL基因表达出现差异，离体培养第3天表达明显提高，一直到第8天还维持较高表达水平，以后外植体褐变的加重，PAL基因表达水平逐渐降低。与对照相比，在Fe盐浓度加倍为55.6mg/L培养基中培养的外植体PAL基因表达水平提高发生的比对照早，培养2天就明显增强，随培养天数延长，一直维持较高的表达水平；其PAL活性也高于对照，两种培养条件下，外植体总酚含量都随着其褐变加重而增加，说明PAL与蝴蝶兰外植体褐变相关。

Abstract:

Semi-quantitative RT-PCR was performed to detect the expression pattern of phenylalanine ammonia-lyase (PAL) gene during Phalaenopsis sp. leaf explant browning. Transcript RNA of PAL was rapidly increased at day 3 after in vitro culture, maintained at a high level until day 8 after culture and then declined. PAL mRNA was present one day earlier than the control after cultured on MS containing 55.6mg/L FeSO4, and PAL activity was higher than the control. The total phenol content in leaf explants on both media increased with browning. It is suggested that PAL gene expression is associated with Phalaenopsis sp. leaf explant browning.