中国主栽香蕉品种和INIBAP引进品种的SSR分析研究

Genetic Diversity of Chinese Main Banana Cultivars (Musa spp.) and Introduced Accessions from INIBAP Using Simple Sequence Repeats (SSRs)

摘要:

利用10对SSR引物对中国14个主栽香蕉品种和从INIBAP引进的33个香蕉品种进行了遗传多样性分析。10个多态性位点共揭示出92个等位基因，每个位点的等位基因数从5到15不等，平均每个位点的等位基因数是9.2，产物片段大小在75bp到310bp之间。用Jaccard系数计算品种间的相似性，相似性数值在0.1到1之间；用UPGMA进行聚类，聚类结果显示，14个主栽品种的遗传变异小，而供试的33个引进品种遗传多样性高。本研究所用的10对引物不能把所有的品种区分开。

Abstract:

Microsatellites were used to investigate the genetic diversity and relationships among 14 Chinese main banana cultivars (Musa spp.) and 33 introduced accessions from International Network for the Improvement of Banana and Plantain (INIBAP). Ten out of 47 microsatellite primer pairs amplified reproducible and discrete DNA bands, revealing a total of 92 alleles. The average number of alleles amplified by per primer was 9.2, ranging from 5 to 15. The sizes of PCR products were between 75bp and 310bp. The genetic similarity among the cultivars was estimated by the Jaccard coefficient，and the similarity value was between 0.1 and 1. Relationships among the cultivars were evaluated using the unweighted pair-grouping with arithmetic average (UPGMA) clustering. The dendrogram showed that the introduced accessions presented higher levels of genetic diversity than the main cultivars. All the cultivars tested could not be discriminated by the 10 primers.