流式细胞术在高等植物研究中的应用

Applications of Flow Cytometry in Higher Plant Research

摘要:

流式细胞术（FCM）是根据所测定的各种细胞性质的不同组合，从细胞群体中把某个亚群分选出来，并对它的功能和形态学进行研究或进一步培养分析。流式细胞术具有快速、灵敏和同时进行多参数检测等优点，对其基本原理和在高等植物中的应用进行了介绍。

Abstract:

Flow cytometry （FCM） is a current technique for sorting certain cell subset from various cellular associations based on differences in cell＇ s physical and chemical properties, by which the selected cell subset could be further studied in function and morphology. The advantages of this technique include rapidity, sensitivity and synchronization of multi-parameter detection. The principles of FCM are briefly described, and its applications in plant cytoplast, protoplast and chromosome are reviewed.