无核荔枝胚胎发育时期蛋白质图谱分析

Proteomic Analysis of Seedless Litchi at Embryo Developmental Stage

摘要:

通过二维聚丙烯酰胺凝胶电泳（2DE）以及计算机辅助的图像分析技术，对荔枝开花后20d的正常与败育胚蛋白质图谱进行了初步分析。结果表明，正常胚总蛋白质斑点数为129，败育胚总蛋白质斑点数为130，其中24个蛋白质点在两种胚中的表达丰度没有明显变化，35个蛋白质点在表达丰度上有明显差异，55％的蛋白则发生了蛋白质缺失、增加以及位置改变等变化。这两种蛋白质组的表达差异说明了胚内蛋白质成分在其败育过程中发生了变化，这些蛋白可能参与了胚败育的调节和控制。

Abstract:

Proteomatic analysis is a new powerful tool for revealing the changes in protein level. Two-dimensional polyacrylamide gel electrophoresis （2DE） and computer image analyses were employed to compare the global protein patterns between normal and aborted embryo of litchi （Litchi chinensis Sonn.）. Totally, 129 and 130 protein spots were obtained from normal and aborted embryos, respectively, of which 24 spots in both kind of embryos showed no significant difference, but 35 spots exhibited obvious differences in quantity, Furthermore, some spots were missing and some new spots emerged in aborted embryo compared with normal embryo. The differences in expressing the proteins between normal and aborted embryos of litchi are useful for regulating and controlling the embryo abortion.