寒兰的快速繁殖技术

Rapid Propagation of Cymbidium kanran Makino

摘要:

以寒兰（Cymbidium kanran Makino）根状茎为外植体，采用B₅ 基本培养基，并附加不同浓度的6-BA、NAA、TDZ（苯基噻二唑基脲-thidiazuron）和S-3307（优康唑．uniconazole），对类原球茎的诱导、继代增殖、分化、生根等进行研究。结果表明：诱导类原球茎的最佳培养基为B₅＋TDZ 0.50 mg L^-1＋NAA 0.25 mg L^-1，诱导率98.3％；继代增殖的最佳培养基为B₅＋S-33071.0 mg L^-1＋NAA0.2 mg L^-1＋蔗糖3.5％，增殖系数9.4：类原球茎分化的最佳培养基为B₅ ＋S.33070.75 mg L^-1＋6-BA 1.0 mg L^-1＋NAA0.4 mg L^-1,分化率87.8％：最佳的生根培养基为1/2B₅＋NAA0.2 mg L^-1＋活性炭0.05％，生根率达100％。

Abstract:

Propagation of Cymbidium kanran Makino by using rhizomes as explants to study the effects of growth regulators on the induction, multiplication and differentiation of protocorm-like bodies （PLBs）, and the rooting. The results showed that the optimal medium for induction of PLBs was B₅ containing 0.50 mg L^-1 TDZ and 0.25 mg L^-1 NAA, in which induction rate of PLBs was 98.3%, for multiplication of PLBs it was B₅ containing 1.0 mg L^-1 uniconazole （S-3307） and 0.2 mg L^-1 NAA and 3.5% sucrose, the multiplication coefficient being 9.4. For differentiation it was best on B₅ medium containing 0.75 mg L^-1 S-3307, 1.0 mg L^-1 6-BA and 0.4 mg L^-1 NAA, in which the differentiation rate was 87.8%. As for rooting, 1/2B₅ supplemented with 0.2 mg L^-1 NAA and 0.05% active carbon, in which rooting rate reached 100%.