蔗糖和多效唑对试管生姜形成的影响

Effects of Sucrose and Paclobutrazol on Microrhizome Formation in Ginger （Zingiber officinale Rosc.）

摘要:

用不同浓度的蔗糖、NAA、Ca₃（PO₄）₂、多效唑（Pac）和光照条件对江西兴国九山生姜（Zingiber officinale Rosc.cv.Jiushan）进行试管姜的诱导，成功诱导出试管姜。结果表明，诱导试管姜的最佳培养基为：MS＋6-BA 0.2 mg L^-1＋NAA 0.5mgL^-1＋Ca₃（PO₄）₂2．5 mg L^-1＋Pac 2．5 mg L^-1＋蔗糖8％；适宜浓度的Pac、Ca₃（PO₃）₃、蔗糖有利于试管姜诱导；NAA和6-BA对试管姜的诱导不起促进作用；延长光照时间有利于试管姜膨大。

Abstract:

Microrhizome of Zingiber officinale Rosc. cv. Jiushan were inducted from tube plantlets, which were derived from explant of stem tip, cultured on MS medium supplemented with 0.2 mg L^-1 BA and varying concentrations of combinations of sucrose, NAA, Ca₃（PO₄）₂, Paclobutrazol （Pac）. The results showed that rhizome production was best in tube culture on MS medium supplemented with 0.2 mg L^-1 6-BA, 0.5 mg L^-1 NAA, 2.5 mg L^-1 Ca₃ (PO₄)₂, 2.5 mg L^-1 Paclobutrazol and 8% sucrose. NAA and 6-BA had no promotion effects on rhizome induction. More illumination stimulatory rhizome growth of the ginger.[