植物绿叶粗蛋白在SDS—PAGE中向负极泳动的富含苯丙氨酸的碱性蛋白的测定

The Determination of A Phenylalanine-rich Basic Protein from Plant Green Leaves Migrated towards the Cathode in SDS-PAGE

摘要:

提取菜心（Brassica campestris L．ssp．chinensis（L．）Makino vat．utillis Tsen et Lee）和生菜（Lactuca sativa L．）绿叶粗蛋白，经SDS-变性后作醋酸纤维素薄膜电泳，证实这两种蔬菜含有分别向负极泳动和向正极泳动的蛋白。菜心绿叶粗蛋白经SDS-PAGE电泳后，从负极电泳缓冲液中获得向负极泳动的蛋白，其苯丙氨酸含量为57.05％，碱性／酸性氨基酸残基的比例为3.04，表明是富含苯丙氨酸的碱性蛋白。

Abstract:

Crude proteins from green leaves ofBrassica campestris L. ssp. chinensis （L.） Makino var. utillis Tsen et Lee and Lactuca sativa L. were subjected to acetate cellulose membrane electrophoresis under SDS-denatured conditions. Two groups of protein were observed, one migrated towards the anode, and the other, the cathode. The cathode protein from Brassica campestris ssp. chinensis vat. utillis was collected from cathode reservoir after SDS-PAGE, and its amino acid components were determined to have 57.0% phenylalanine, the ratio of basic/ acidic amino acid residues was 3.04, indicating the protein towards cathode was a phenylalanine rich basic protein. It must be emphasized that the protein migrated towards the cathode had never been reported previously.