厚荚相思树根瘤菌HJ06菌株的16S rDNA全序列和nifA基因片段分析

16S rDNA and nifA Gene Sequence Analysis of Rhizobium Strain HJ06 Isolated from Acacia crassicarpa

摘要:

对厚荚相思（Acacia crassicarpa）根瘤菌HJ06菌株的16S rDNA全序列利nifA基因片段进行了测定。结果表明，HJ06菌株在以16S rDNA序列构建的系统发育树状图中位于根瘤菌属（Rhizobium）分支中，与根痛菌属各个种的相似性达95％以上；从HJ06菌株克隆出的585 bp nifa基因片段与Klebsiella pneumoniae的同源性达到99.3％，与Klebsiella oxytoca的NifF，NifL，NifA，NifB蛋白基因的同源性为97．8％。

Abstract:

The determination of full-length 16S rDNA sequence and nifA gene segments of root nodule bacterium strain HJ06 isolated from Acacia crassicarpa showed that strain HJ06 had above 95% similarity with the genus Rhizobium. The nifA gene segments of HJ06 have 99.3% isogenesis with nifA genes of Klebsiella pneumoniae and 97.8% isogenesis with genes ofNifF, NifL, NifA and NifB proteins of K. oxytoca.