建兰与纹瓣兰种间杂种胚培养研究

Embryo Culture of Hybrid between Cymbidium ensifolium and C. aloifolium

摘要:

采用人工授粉的方法，以‘小桃红’建兰为母本、纹瓣兰为父本进行杂交，获得了种间远缘杂交后代植株。杂交果苹与母本自交果荚的大小差异大小，但鲜重明显大于母本，果荚内的种子量却少于母本。杂种胚离体培养90 d出现绿色原球胚，210 d可萌发出大量植株；而‘小桃红’自交种子培养210 d只形成少量绿色根状茎。杂种胚的萌发具有杂种优势，不仅萌发快，而且出苗率高，萌发时先形成原球胚，原球胚上长出大量小根毛，继而形成大量假鳞茎，假鳞茎经转瓶培养后，分化形成大量健壮植株，分化率高。

Abstract:

Interspecific F1 hybrid between Cymbidium ertsifolium ‘Xiao Tao Hong＇ and C. aloifolium （L.） Sw. were obtained by pollination. The fresh weight of fruits of F₁ hybrid was higher than that of selfed C. ensifolium ‘Xiao Tao Hong＇ , but seeds of the former were less than the latter. Green protocom-like bodies appeared after 90 days, from hybrid embryos cultured on MS medium supplemented with 170 mg L^-1KH₂PO₄, 1 mg L^-1 BA and 50 g L^-1 banana, and formed a number ofplantlets after 210 days. However, only a few rhizomes occurred from seeds of selfed ‘ Xiao Tao Hong＇ cultured after 210 days. Embryo culture of the hybrid had the advantage of fast growth and high plantlet formation rate.