盾叶薯蓣试管株芽的诱导

The Induction of Bulbils of Dioscorea zingiberensis

摘要:

以盾叶薯蓣（Dioscorea zingiberensis）试管植株为材料，选取带芽茎段为外植体，转接到株芽诱导培养基上15 d后，原茎段基部开始产生株芽突起，30 d后每一茎段可产生3-5个已生根的株芽，株芽诱导率为100％，株芽诱导数为180个／40株，其移栽成活率可达90％以上。株芽形成的适宜培养条件：温度为26±2℃，光照时间14 h d^-1，光照强度为1500-2000l x：适宜培养基组成为：MS＋6-BA 4.0 mg L^-1＋IBA 1.0 mg L^-1＋蔗糖6％-9％＋活性炭0.5％＋琼脂7％。离体诱导的盾叶薯蓣试管株芽能直接发育为新植株，为盾叶薯蓣的快繁提供了一种新的方法。

Abstract:

An in vitro protocol for rapid propagation of medicinal plant Dioscorea zingiberensis C. H. Wright was optimized. Nodal segments with buds cultured obtained from vessels were used as explants which were the ncultured on bulbil induction medium. The explants started to appear emergence from basal segments after 15 days. Three to five bulbils with roots developed from each segment were obtained after another 15 days. The optimal culture conditions for bulbil formation were 26±2℃ at 1 500-2 000 lx illuminated for 14 hours per day on MS medium supplemented with 4.0 mg L1 BA and 1.0 mg L^-1 IBA in addition of 6%-9% sucrose, 0.5% active charcoal, and 7% agar. The bulbil induction rate was 100%, and the plantlet survival rate being above 90%.