微囊藻毒素合成酶基因的PCR检测方法

A PCR Method for Detection of Microcystin Synthetase Gene

摘要:

针对微囊藻毒素合成酶基因簇的核酸序列，筛选特异性引物，探索一种适用于自然水样中微囊藻产毒潜能检测的全细胞PCR方法。经灵敏度测试表明，这种PCR方法的检测下限相当于100 cells。该方法不需要提取基因组DNA，检测所需水样量少，具有操作简便、快速、成本低、灵敏度高等优点，能应用于水库等饮用水源水体中具有产毒潜能的微囊藻的检测。

Abstract:

Based on the sequences of racy genes from Microcystis, a specific primer pair was determined. PCR-mediated detection of mcyB gene was developed and applied to the identification of potential microcystin produced from Microcystis strains in natural water bodies. The sensitivity of PCR method was investigated, and the detection limit was as few as 100 cells per reaction. The method does not require extraction of the genomic DNA of Microcystis, and does not need large volume of water samples. It is simple, sensitive, rapid and economical, which can be used for the detection of the toxic Microcystis strains in water sources for human consumption.