水稻内生固氮细菌的分离及鹑鸡肠球菌在水稻根中的分布

Isolation of an Endophytic Diazotroph from Rice and the Distribution of Enterococcus gallinarum in Rice Roots

摘要:

从福建省推广的谷杆两用水稻201中分离出内牛细菌12株，鉴定为阴沟肠杆菌（Enterobacter cloacae）、鹑鸡肠球菌（Enterococcus gallinarum）、短小芽孢杆菌（Bacillus pumilus）、地衣芽孢杆菌（Bacillus licheniformis）、巨大芽孢杆菌（Bacillus megaterium）、球形芽孢杆菌（Bacillus sphaericus）、蜡状芽孢杆菌（Bacillus cereus）、脂肪杆菌（Pimelobacter）和节杆菌（Arthrobacter）。选择固氮酶活性较大的3个菌株，对其抗性、革蓝氏染色特性进行了研究。采用三亲交配法将标记基因nifH-lacZ导入到鹑鸡肠球菌中，用该固氮细菌回接水稻，对感染鹑鸡肠球菌的水稻根进行β-半乳糖苷酶组织化学染色、光学显微镜和电子显微镜观察。结果表明，固氮细菌鹑鸡肠球菌在水稻根表皮细胞、内皮层细胞、维管组织细胞和细胞间隙中存在。

Abstract:

Twelve strains of endophytic diazotrophic bacteria were isolated from grain-straw-dual-purpose rice 201 popularized in Fujian Province. They were identified as： Enterobaeter cloacae, Enterococcus gallinarum, Bacillus purnilus, B. licheniformis, B. megaterium, B. sphaericus, B. cereus, Pirnelobacter, and Arthrobacter. Three strains with high acetylene reduced activity were selected to study the bacterial resistance to antibiotics, and results of Gram staining. The plasmids harboring the reporter gene lacZ fused to the promoters of nifH were transformed into Enterococcus gallinarum by triparental crossing. The inoculated rice roots were examined by β-galactosidase staining and observed under light and electron microscopes. The results indicated that the endophytic diazotrophic bacteria existed in epidermal cells, cortex cells, vascular tissue cells and intercellular spaces office roots.