乙烯利、ACC、AOA和AgNO3对绿豆下胚轴插条不定根形成的作用

Effects of Ethephon, ACC, AOA and AgNO3 on Adventitious Root Formation in Mung Bean Hypocotyl Cuttings

摘要:

以绿豆下胚轴插条为实验材料,研究了乙烯利、ACC、AOA和AgNO₃对其不定根形成的影响。结果表明:乙烯利和ACC能促进绿豆下胚轴插条的生根,最适浓度分别为50 μmol/L和10 μmol/L;AOA和AgNO₃ 明显抑制不定根形成,随浓度增加,抑制作用增强。插条离体后24 h内对ACC的促进作用和AOA的抑制作用敏感。插条在0-6 h和18-24 h用ACC处理,在0-2 h和22-24 h用50 μmol/L乙烯利处理的生根效果好。乙烯在不定根形成的诱导期和起始晚期起促进作用。

Abstract:

Effects of ethephon, 1-aminocyclopropane-1-carboxylic acid (ACC), aminioxyacetic acid (AOA) and AgNO₃ on adventitious rooting in mung bean hypocotyl cuttings were studied. Results indicated that ethephon and ACC promoted adventitious root formation in mung bean hypocotyl cuttings, the optimal concentrations being 50 μmol/L and 10 μmol/L respectively, whereas AOA and AgNO₃ significantly inhibited adventitious rooting, and the effect of which were dose-dependent. The promoting effect of ACC and the inhibitory effect of AOA on the cutings excised after 24 hours were sensitive．The rooting efects were best in treatment with 10 μmol/L ACC for 0-6 hours an d 18-24 hours， and in treatment with 50 μmol/L ethephon for 0-2 hours and 22-24 hours．It is concluded that the promoting efects of ethephon and ACC on adventitious rooting were at induction stage and late initial stage．