细裂银叶菊叶的组织培养繁殖研究

In vitro Propagation of Senecio cineraria cv. Silver Dust

摘要:

以细裂银叶菊叶片为材料,进行愈伤组织的诱导、分化培养及生根诱导培养。结果表明:叶片愈伤组织的诱导以MS+2,4-D 2 mg L^-1+BA 1 mg L^-1+NAA 0.1 mg L^-1培养基较好;分化培养以MS+BA 0.5 mg L^-1+NAA 0.1 mg L^-1为好;生根诱导以1/2MS+NAA 0.01 mg L^-1效果最好。

Abstract:

The leaves of Senecio cineraria cv. Silver Dust were used to study the callus induction, shoots induction and rooting. It was shown that MS medium supplemented with 2 mg L^-1 2,4-D+1 mg L^-1 BA+0.1 mg L^-1 NAA was good for callus induction, while MS medium containing 0.5 mg L^-1 BA+0.1 mg L^-1 NAA, half-strength MS medium containing 0.01 mg L^-1 NAA gave best results for shoot induction and for rooting, respectively.