阳桃胚乳愈伤组织诱导和不定芽发生的研究

Callus Induction and Plant Regeneration from Endosperm of Averrhoa carambola

摘要:

首次成功建立阳桃胚乳组织培养并获得胚乳再生植株。胚乳愈伤组织诱导以培养基MS+2,4-D 2.0 mg L^-1 +BA 0.2 mg L^-1的效果最好,诱导频率可达94.7%,愈伤组织乳白色,结构致密,生长旺盛;将其接种在培养基MS+ZT 3.0 mg L^-1 +NAA 0.2 mg L^-1上,愈伤组织由乳白色致密型转变为淡绿色致密型,进而形成绿色芽点,分化出不定芽,分化频率可达73.3%;胚乳植株在培养基MS+ZT 2.0-2.5 mg L^-1 +NAA 0.05 mg L^-1上进行壮苗和营养繁殖。

关键词:阳桃; 胚乳; 离体培养

Abstract:

Explants from endosperm of Averrhoa carambola were used to establish culture in vitro and regeneration system. The experiments showed that endosperm inoculated on MS medium supplemented with 2.0 mg L^-1 2,4-D and 0.2 mg L^-1 BA, gave highest callus induction rate (94.7%). The callus was milk white and compact. When the endosperm derived callus was cultured on MS medium containing 3.0 mg L^-1 ZT and0.2 mg L^-1 NAA, the callus became greenish and compact, the shooting frequency being 73.3%. Optimal medium for multiplication of plantlets was MS supplemented with 2.0-2.5 mg L^-1 ZT and 0.05 mg L^-1 NAA.

Key words:Averrhoa carambola; Endosperm; Tissue culture