氨基茚磷酸(AIP)和氨基氧乙酸(AOA)对茉莉酸甲酯诱导的烟草一些酶活性的影响

Effects of Aminoxyacetic Acid (AOA) and 2-Aminoindan-2-phosphonic Acid (AIP) on the Activities of Some Enzymes Induced by Methyl Jasmonate in Tobacco Calli

摘要:

用茉莉酸甲酯(MJ,1 mg ml^{-1)处理培养在含0.1 mmol/LAIP(水杨酸合成抑制剂)和/或1 mmol/LAOA(乙烯合成抑制剂)的MS培养基上的烟草愈伤组织,测定某些酶的活性。结果表明:MJ明显提高过氧化物酶(POD)、β1,3-葡聚糖苷酶和几丁质外切酶的活性,略微促进苯丙氨酸解氨酶(PAL)、多酚氧化酶(PPO)的活性,抑制几丁内切酶的活性,而AOA和AIP则明显抑制MJ对POD和β1,3-葡聚糖苷酶活性的诱导作用,但对MJ诱导的PAL和PPO的活性影响很小,AOA和AIP可能作为逆境因子促进PAL的活性。AOA能部分解除MJ对几丁内切酶的抑制作用,但对MJ诱导的几丁外切酶的影响较小,而AIP抑制几丁内切酶的活性,也抑制MJ对此酶的诱导作用。因此我们认为:MJ对POD、PPO和几丁内切酶的影响可能是通过乙烯途径,对β1,3-葡聚糖苷酶和几丁外切酶的影响可能是通过水杨酸(SA)途径,而对PAL的影响可能是通过其它途径。}

Abstract:

Tobacco calli, induced from leaf explants on MS medium supplemented with 0.2 mg L^{-1 NAA, 0.2 mg L-1 kinetin and 1 mg L-1 2,4-D, were cultured on MS medium containing 0.1 mmol/L 2-aminoindan-2-phosphonic acid (AIP, inhibitor of phenylalanine ammonia lyase) or 1 mmol/L aminoxyacetic acid [AOA, inhibitor of ACC(1-aminocyclopropane-1-carboxylic acid) synthase], or 0.1 mmol/L AIP and 1 mmol/L AOA in combination. The calli were then treated with 1 mg ml-1 methyl jasmonate (MJ) to determine the activities of peroxidase (POD)，phenylalanine ammonia lyase(PAL)，β l,3-glucanase，polyphenol oxidase，and chitinase．The result showed that MJ markedly increased the activities of POD，β l,3-glucanase，exochitinase and slightly increased the activities of PAL and polyphenol oxidase(PPO)，while it inhibited the activity of endochitinase．AOA and AII)significantly inhibited the induction effect of MJ on the activities of POD and β 1,3-glucanase，but the induction effect of MJ on PAL and polyphenol activities was less． AOA could partially relieve the inhibition effect of MJ on endochitinase, but had less effect on MJ-induced exochitinase activity．AIP inhibited the activity ofendochitinase and the induction effect of MJ as wel1．It is inferred that the efrect of MJ on POD, PPO and endochitinase may be through the signal transduction of ethylene，and on β 1,3-glucanase and exochitinase through salicylic acid, while on PAL there exists another pathway.}