发育中玉米胚结合蛋白(BiP)的动态变化及定位

Changes and Localization of Binding Protein (BiP) during Maize Embryo Development

摘要:

Westernblot检测表明,在玉米胚发育过程中结合蛋白(BiP)含量与胚可溶性蛋白含量变化一致,在授粉16 d后BiP含量随发育而增加;对热激不敏感。组织化学免疫定位表明,在玉米胚发育的不同时期,BiP主要定位在胚芽端、初生维管组织和糊粉层中,提示胚在构建器官的同时,也为其功能执行准备了条件;热激不影响其定位。

Abstract:

Western blot showed that binding protein(BiP) kept a high expression level during maize embryo development, which coincided with the change in embryo soluble protein. The content of soluble protein rapidly increased with the embryo development after pollination for 16 days, while BiP markedly increased from 22^nd day after pollination. The BiP failed to respond to heat shock. Immunohistochemical localization showed that BiP localized in plumule, primary vascular tissue and aleurone layer during embryo development.