蝎尾蕉属植物的组织培养与快速繁殖

In vitro Propagation of Heliconia Plants

摘要:

以蝎尾蕉属植物的吸芽为外植体,经灭菌处理后接种到MS+BA 2-10 mg L^-1+NAA 0.2-1.0 mg L^-1培养基上,能进行不定芽的诱导和增殖,不同品种的增殖倍率相差较大,所需最适BA的浓度也不同。生根培养基以MS+NAA 0.5 mg L^-1+0.5%活性炭的效果较好。以沙,或泥炭土:沙=1:1,或珍珠岩:河沙=1:1三种基质移栽的试管苗,成活率达到90%以上。

Abstract:

New buds obtained by dark culture from the rhizomes of ten species or cultivars and hybrids of Heliconia were used as explants which were cultured on MS medium supplemented with 2-10 mg L^-1 BA and 0.2-1.0 mg L^-1 NAA. The experiments showed that the explants could induce shoots and proliferate. Best result for rooting was observed on MS medium supplemented with 0.5 mg L^-1 NAA plus 0.5% active carbon. The plantlets transferred to pots containing sand/turf mixture (1:1), or sand/perlite (1:1), or sand only showed a survival rate more than 90%.