小麦与高冰草不对称体细胞杂种F_5代部分株系的根尖细胞染色体分析

Chromosome of Root Tip Cells in F_5 Plants Derived from Asymmetric Somatic Hybrids between Triticum aestivum and Agropyron elongatum

摘要:

将普通小麦“济南177”(Triticumaestivumcv.Jinan177)原生质体(受体)和经紫外线照射的高冰草(Agropyronelongatum)原生质体(供体)用PEG(聚乙二醇,polyethyleneglycol)法诱导融合,获得外形偏向小麦的不对称体细胞杂种及后代。经过田间繁育,现已到F5代。对来源于同一个体细胞杂种克隆的不同株系(Ⅱ-2,8-1,Ⅱ-Ⅰ-8)的F₅代的根尖细胞染色体进行核型分析,并与其亲本比较,结果表明,杂种株系染色体的形态、数目在遗传上均趋于稳定。各杂种株系的染色体与亲本小麦“济南177”之间有多处显著的不同,杂种株系之间也存在差异,推测高冰草的染色体小片段可能进入了受体小麦的染色体中。

Abstract:

Asymmetric somatic hybrids having wheat appearance were obtained from protoplast fusion of Triticum aestivum L cv. Jinan 177 (as acceptor) and UV irradiated A gropyron elongatum (as donor) using PEG (polyethylene glycol) method. The karyotype analysis was made in root tip of F₅ of different lines (Ⅱ-2,8-1,Ⅱ-Ⅰ-8) from a somatic hybrid, and was compared with the parent wheat cultivar Jinan 177. The results indicated that the hybrids were basically stable in chromosome morphology and number. However, some differences in chromosome morphology were observed between the hybrids and the parent, as well as among the three hybrids, suggesting that small chromosome fragments from Agropyron elongatum were integrated into the chromosomes of the acceptor (wheat).