低温胁迫下氯丙嗪和氯化镧对水稻幼苗脯氨酸积累的影响

Effects of Chlorpromazine and Lanthanum Chloride on Proline Accumulation in Rice Seedlings under Cold Stress

摘要:

低温胁迫下水稻幼苗质膜透性增大、相对含水量(RWC)下降、丙二醛(MDA)含量增加,同时脯氨酸(Pro)积累。以氯丙嗪(CPZ)和氯化镧(LaCl₃)对水稻幼苗预处理以阻碍Ca²⁺·CaM(钙调素)信使传导后,加剧了低温胁迫下水稻幼苗质膜透性的增加、RWC的下降、MDA含量的增加和Pro的积累,说明水稻幼苗受伤害程度将加重。

Abstract:

The roots of 13-day-old rice seedlings were pretreated with 50 μmol/L chlorpromazine or 500 μmol/L lanthanum chloride (LaCl₃) at 25℃ for 15 h to determine the changes in relative water content (RWC), malondialdehyde (MDA) content, relative permeability of cell membrane, and proline (Pro) accumulation in the seedlings under cold stress at 6-7℃ and light intensity at 200 μmol m^-2s^-1. The experiments showed that the RWC decreased, relative permeability of cell membrane and MDA content increased, meanwhile, the proline was accumulated under cold stress. Such trends were enhanced significantly in the seedlings pretreated with chlorpromazine or LaCl3 under cold stress.