沙田柚黄龙病病原体的电镜观察与PCR检测

Detection of Citrus Yellow Shoot Pathogen in Shatianyou Pommelo by Electron Microscopy and PCR Method

摘要:

柑桔黄龙病可以侵染沙田柚而产生典型的‘斑驳’症状。我们通过电镜观察表明,带病沙田柚的病原体在形态、大小和构造上均与柑桔黄龙病病原体(类细菌)相似;PCR扩增也证明其病原体DNA与柑桔黄龙病病原体的PCR产物具有很高的同源性。

Abstract:

Citrus yellow shoot disease (citrus huanglongbing) leaves of Citrus maxima cv. Shatianyou collected from pommelo orchard in Meizhou, Guangdong Province, showed typical symptom of leaf mottling. The pathogen which was observed under electron microscopy was similar in morphology and structure to bacteria-like organism of citrus huanglongbing disease. Polymerase chain reaction (PCR) analysis also proved that the DNA of the pathogen was greatly homologous with PCR product from that of citrus huanglongbing pathogen. The result exhibits that the citrus yellow shoot could infect pommelo, which seldom appeared in this fruit.