红麻及其近缘种的RAPD分析

Random Amplified Polymorphic DNA (RAPD) Analyses among Hibiscus cannabinus and Related Species

摘要:

利用随机扩增多态性DNA(RAPD)技术分析了木槿属(Hibiscus)Furcaria组中纤维作物红麻(H.cannabinus)及其6个近缘种植物的25份材料。用筛选出的16个引物扩增出192个RAPD条带,它们表现出丰富的多态性。根据得到的RAPD指纹图谱,计算其Nei氏相似系数和遗传距离,并构建了它们的系统树。结果表明:25份材料可划分为7个组,H.penduriformis和H.calyphyllus两个种为一组;红麻种分为两个组,一组为栽培品种,另1组为野生型材料;其余4个种各成为1组。玫瑰麻(H.sabdariffa)和金线吊芙蓉(H.radiatus)的关系较近,而且两者与红麻的亲缘关系也较近,而其它四个种与红麻的关系较远。H.trionum与其它六个种的关系较远。

Abstract:

RAPDs were used to study the relationships among species in Hibiscus sect. Furcaria. Twenty-five accessions of 7 species we re examined. Sixteen primers of arbitrary sequences were screened from 80 primer s for DNA amplification. A total of 192 bands were generated, of which 149 bands were polymorphic markers. Dendrogram was constructed using Nei's genetic simila rity value and MINTS program. The result showed that 25 accessions were clustere d in 7 clades, H. penduriformis and H. calyphyllus formed a clade,and 15 accessions of H．cannabinus were clustered in two clades，one being the cultivars，and the other，the wild type materials．The rest 4 species formed 4 individual clades．The most parsimonious dendrogram shows that H．radiatus is closely related to H. sabdarifa．The closely related species to the cultivars of H.cannabinus are H．sabdarifa and H．radiatus, whereas H．trionum appears to be highly diverged from all the other taxa．