杜氏藻属四个种的核型分析

The Karyotypes of Four Dunaliella Species (Algae)

摘要:

采用染色体分带方法对杜氏藻属（Dun>0.25aliella）4个种的核型进行分析。经过25℃12 h d（-1）的光周期诱导,杜氏藻属4个种出现同步化生长。用0.05％秋水仙素处理,再经低渗、固定和高位（60 cm）滴片,获得杜氏藻属4个种的核型。结果表明:杜氏藻属4个种都是单倍体,其染色体大多为短杆状、极小。染色体数分别是:D.salina n=13,D primolecta n=20,D.bardawil n=10,D.parvan=16。它们大都可见较明显的初级缢痕,且都在染色体的中部。

Abstract:

The karyotypes of four species of Dunaliella were analyzed using Giemsa staining method. The organisms were cultured in Ben-Amotz medium with 1.5 mol/L NaCl at 25℃ in a 12/12 h light-dark cycle for three days to induce synchronous growth of the algae. The karyotypes were observed after a series of treatments including pretreatment with 0.05% colchicine for 12-16 h, hypoosmotic shock, fixation, and dropping from 60 cm high to glass slide for breaking the surface coat of Dunaliella cells. All species observed are haploid with short and bacilliform chromosomes． The chromosome numbers of Dunaliella species are n=13 in D．salina，n=20 in D．primolecta， n=l0 D．bardawil，and n=l6 D．parva．All the chromosomes are wim median centromere, and the primary constrictions were obvious.