硝酸银对桑树遗传转化的作用(简报)

The Effect of Silver Nitrate on Genetic Transformation in White Mulberry (Morus alba L.)

摘要:

调查了硝酸银对农杆菌介导的桑(MorusalbaL.)品种“新一之濑”遗传转化的作用。在抗性筛选培养基中添加2 mgL^-1硝酸银,桑叶盘褐化死亡率减少7.2%,抗性芽分化率增加3.9%,外源基因转化频率增加9.6%,“假阳性”转化体减少42.5%。感染农杆菌的桑叶盘经MS培养基培养、农杆菌液体培养和固体培养基上划线培养等实验证实,硝酸银对工程农杆菌LBA4404的生长具有抑制作用,这可能是提高桑树转化频率的原因之一。

Abstract:

To determine the effect of silver nitrate on genetic transformation, Agrobocrerium tumefaciens-mediated 1eaves of Morus alba cv. Shiniehinose were used as explants cultured on kanamycin-resistant screening medium (MS basal medium supplemented with 200-400 mg L^-1 carbenicillin and 30-40 mg L^-1 kanamycin)，to which 2 mg L^-1 silver nitrate was added．The results showed that the rate of browning leaf discs infected with Agrobacterium tumefaciens strain LBA4404 decreaced by 7.2％ ，regeneration frequency of kanamycin-resistant buds increased by 3.9％ , transformation frequency of Gly gene increased by 9.6％, and the false positive transformants as detected by PCR decreased by 42.5％．The experiments demonstrated that silver narrate had inhibiting effect on the growth of strain LBA4404，which might increase transfonnation frequency in white mulberry.