四种观赏凤梨的离体繁殖

In vitro Culture of Some Bromeliaceae

摘要:

以粉菠萝[Aechmeafasciata(Lindl.)Baker]、八宝剑(VrieseapoelmaniiHort.)、虎斑姬凤梨[Cryptanthuszonatus(Vis.)Beer]、七彩羞凤梨[Neoregeliacarolinae(Beer)L.B.Sm.]等的幼株茎段为外植体,以MS+NAA 0.5 mgL^-1+BA 5 mgL^-1为诱导培养基,MS+BA 1 mgL^-1+NAA 0.1 mgL^-1 为增殖培养基,可获得较好增殖效果。用1/2MS+NAA 0.5 mgL^-1生根效果较好,以椰糠:砂=1:1,或椰糠:泥炭土:蛭石=1:1:1为基质移苗的成活率均达95%以上。

Abstract:

Micropropagation was studied of four brometiad plants Aechmea fasciata，Vriesea poelmsnii, Cryptanthus zonatus and Neoregelia carolinae, cultured by stem explants．Induction medium of MS+NAA 0.5 mg L^-1+BA 5 mg L^-1 led to budding after one or two weeks of culture． MS+BA l mg L^-1 +NAA 0.l mg L^-1 was the optimum medium in subculture． The optimum concentration of NAA was 0.5 mg L^-1 in rooting. Plantlets transplanted oil media contained coconut-bant:sand=1：l or coconut-bant：turf：vermiculite (1：1：1) had better surviva1 rate up to 95％ ．