转bar基因水稻在杂种优势育种中的利用

APPLICATION OF BAR-TRANSGENIC RICE IN BREEDING FOR HETEROSIS

摘要:

以3个美国转bar基因水稻抗除草剂品种Bengal Hu-10、Cypress PB-6和Gulfmont 为父本,分别与三系、两系不育系及人工去雄恢复系杂交。考种结果发现:这3个抗性亲本所配组合杂种优势不明显。通过杂交将抗除草剂亲本中的bar基因转移到常规恢复系,已经培育出3个抗除草剂恢复系明恢63-B、测64-B和特青-B;且已选配出5个抗性杂交组合,它们能保持原组合的产量水平。还讨论了水稻杂种优势利用面临的问题和转bar基因水稻抗性亲本在杂种优势上的应用前景。

Abstract:

Three bar-transgenic rices，Bengal Hu-10，Cypress PB-6，and Gulfmont as male parents，were crossed with 6 male sterile lines：Zhenshan 97A, Bo A，Pei-ai 64S，Nan xiang 95S，Xi 217S and GD 1-S. Eight restorers emasculated were crossed with the three herbicide-resistant sources．Forty-eight F₁ hybrids were obtained totally The results indicated that heterosis in these F₁ hybrids were not obvious By transferring herbicide (Liberty)-resistant bar gene into conventiona1 restorer 1ines, 3 restorer 1ines (Minghui 63-B，Ce 64一B．and Teqing-B)were developed via crossing，and 5 resistant combinations，i.e Zhenshan 97A／Minghui 63-B (B₂F₁-B), Zhenshan 97A／Ce64一B(B₂F₂-G)．Zhenshan 97A／Ce 64-B(B₃F₁-C)，Pei-ai 64S／Teqing-B(B₁F₂-C)．and Pei-ai 64S／Teqing-B(B₂F₁-B)，had been bred，which retained the same yielding level oftheir original combinations．The problems ofheterosis application，and the prospect of bar-transgenic rice parents in breeding for heterosis are discussed.