柚类品种遗传相互关系的RAPD标记研究

GENETIC RELATIONSHIPS AMONG CULTIVARS OF CITRUS MAXIMA (BURM.) MERR. USING RAPD MARKER TECHNIQUE

摘要:

利用22个经筛选在样品间具多态性的10碱基随机引物,对柚 [Citrus max ima (Burm.) Merr.] 的主要品种及柚的一个野生近缘种云南红河橙 (C. hongh eensisY. M. et al.)、一个栽培近缘种葡萄柚 (C. × paradisi Macfad) 共79个样品的总DNA进行PCR扩增。在样品间共产生164条RAPD标记带,其中多态性带134条,多态性百分率为81.71%;以NTSYS-pc (Version 1.80)软件计算样品间的Jaccard遗传相似系数,并进行UPGMA聚类分析。结果表明野生近缘种云南红河橙及栽培近缘种葡萄柚与柚三个种种内聚合良好,种间分异明显,为柚分类上的地位奠定了遗传学基础。特异性RAPD分子标记的研究为柚类品种的鉴定提供了重要指标。

Abstract:

The relationships among cultivars of Pomelo [Citrus max ima (Burm.) Merr.] and two related species, Grapefruit (C.× paradisi Macfad) and Honghe Papeda (C. hongheensi．Y．M．et al.)，were investigated from 79 samples, which were probed using RAPD technique and statistical anaiysis． Twenty-two random 10-base primers were screened for the study． They produced 164 RAPD marker bands among 79 accessions, in which 134 RAPD bands were polymorphic．The rate of polymorphic RAPD bands was 81.71％．Using NTSYS-program and the method of UPGMA, Jaccard genetic similarity coefficient among accessions was calculated, and the dendrograms were constructed． The results show that three species correspond with three clusters of UPGMA dendrogram, supporting the species stallls of pomelo．Some cultivar-specific RAPD markers are found as important markers for pomelo identification．