番木瓜两性基因的RAPD标记

RAPD MAKERS FOR HERMAPHRODITE GENE IN PAPAYA

摘要:

应用混合分离群体分析法,在205个10碱基随机引物中,寻找番木瓜两性基因(M₂)的RAPD标记;发现两个多态性RAPD条带:OPQ07₁₈₀₀和OPE06₁₀₅₀,它们仅能在两性DNA池中扩增到,而不能在雌性DNA池中扩增到。用上述两引物检测了210株番木瓜单株DNA,结果表明,OPQ07₁₈₀₀和OPE06₁₀₅₀与M2基因紧密连锁,它们位于M₂基因的两侧,其遗传距离分别为4.5和1.9 cM。

Abstract:

Bulked segregant analysis was used to identify RAPD markers linked to the hermaphrodite gene (M₂) in papaya (Carica papaya L.). DNA bands amplified from 205 random 10-mer primers were screened. Two polymorphic bands, OPQ-07₁₈₀₀ and OPE-06₁₀₅₀, were found, which were present in the hermaphrodite bulked DNA sand absent in the female bulked DNAs. 210 individual papaya plants were then tested with these two primers. The results indicated that the RAPD markers, OPQ-07₁₈₀₀ and OPE-06₁₀₅₀, were tightly linked to M₂, which located at both sides of M₂, and have a map distance of 4.5 cM and 1.9 cM, respectively.