水稻广亲和品种核DNA cosm id文库的构建和鉴定

THE CONSTRUCTION AND CHARACTERIZATION OF GENOMIC COSMID LIBRARY FOR RICE NUCLEAR DNA OF A WIDE COMPATIBLE VARIETY

摘要:

用改进的方法成功构建了一个高质量的水稻广亲和品种(Cpslo17)的核基因组cosmid文库。文库以SuperCos1为载体,克隆总数约为12万以上,克隆平均插入片段约40kb,空载率约为5%,文库容量覆盖水稻单倍基因组约9倍以上。用RFLP标记23D12R作为探针,从文库中分离到一个位于水稻广亲和基因座S5附近的克隆R2I19。

关键词:水稻; 广亲和品种; cosmid文库

Abstract:

A genomic cosmid library of the wide-compatibility-variety rice (Oryza sativa L.Cpslo17) had been generated using vector SuperCos1. The library containing more than 1.0×10⁵ recombinants with an average insert size of approximately 40 kb represented more than 9 times haploid genomic equivalents, but 5% clones did not carry any insert. Using RFLP marker 23D12R linked tightly to S₅ as probeto screenlibrary, a new clone R2I19 was isolated and determined.

Key words:Rice; Wide compatible variety; Cosmidlibrary