龙须藤凝集素的分离纯化和性质

PURIFICATION AND CHARACTERIZATION OF LECTIN FROM BAUHINIA CHAMPIONII BENTH.

摘要:

利用酸化处理的Sepharose 6B亲和柱从龙须藤（Bauhinia championii)种子中分离纯化出龙须藤凝集素（BCL），其比活性比抽提液提高了57倍，活力回收率达63.3％。经Sphadex G-100测得BCL的分子量为64 000，SDS－PAGE的结果表明BCL由两个相同的亚基组成，亚基分子量为32 000，等电聚集凝胶电泳测得其等电点为4.70。BCL是一种糖蛋白，其中性糖含量为3.0％。N－乙酰－D－氨基半乳糖能强烈地抑制BCL对兔红细胞的凝集作用。

关键词:凝集素; 龙须藤; 分离纯化

Abstract:

Bauhinia championii lectin (BCL) was purified from the seeds of Bauhinia championii Benth. by affinity chromatography on an acid-treated Sepharose 6B column. Hemagglutinating activity of the purified BCL increased by 57 times and the activity recovery was 63.3%. The molecular weight of BCL is 64000 consisting of two identical subunits. The subunit molecular weight is 32000 with isoelectric point of 4.70. BCL is a glycoprotein containing 3.0% of sugar. N-acetyl-D-galactosamine was determined to be a most potent inhibitor of BCL in agglutinating rabbit erythocytes.

Key words:Lectin; Bauhinia championii; Isolation and purification