Cu2+作用下辣椒膜脂过氧化及倍半萜环化酶基因转录

THE EXPRESSION OF SESQUITERPENE CYCLASE GENE AND LIPID PEROXIDATION UNDER THE TREATMENT OF Cu2+ IN DETACHED LEAVES OF CAPSICUM ANNUUM

摘要:

分析了在CuCl₂作用下辣椒叶片倍半萜环化酶活性，倍半萜环化酶mRNA表达，细胞GSH和GSSG代谢及膜脂过氧化，结果表明，Cu²⁺能诱导辣椒叶片表达倍半萜环化酶活性，酶活性的表达与相应的基因转录有关，辣椒叶片在CuCl₂作用下合成了含－SH的螯合肽，相应地GSH含量下降，GSSG的含量有所上升，细胞膜过氧化作用加剧，推测细胞膜脂过氧化产物或GSH氧化产物可能参与了CuCl₂诱导辣椒倍半萜环化酶基因表达的信号传递作用。

Abstract:

An analysis was made on expression of sesquiterpene cyclase gene, glutathione reduced and oxidized forms (GSH, GSSG), and lipid peroxidation and their corelationship in the detached leaves of Capsicum annuum treated with CuCl₂. The results showed that sesquiterpene cyclase gene was induced to express by elicitation of CuCl₂. The content of phytochaletin and GSSG increased, whereas GSH decreased in leaves under the elicitation of CuCl₂, and accumulation of lipid peroxidation products increased accordingly. It is speculated that solue kind of lipid peroxidatiou products or oxidation products of GSH might involved in the signal cascades in the elicitation of sesquiterpene cyclase gene expression under CuC1₂ treatment．