大花飞燕草的组织培养及再生体系建立

TISSUE CULTURE AND ESTABLISHMENT OF REGENERATION SYSTEM OF DELPHINIUM GRANDIFLORUM

摘要:

分别采用种子切段、无菌苗真叶作外植体首次成功建立了大花飞燕草的组织培养和再生体系。结果表明:大花飞燕草种子切段和无菌苗真叶大多数是通过愈伤组织途径再生,也有极少数不经过愈伤组织阶段而直接再生出小植株。在合适的培养基上,种子切段和叶片两种外植体离体培养均能高效再生,平均每个外植体能分化出10-20个不定芽.种子切段培养的最适通用培养基为改良MS附加ZT 3 mg L^-1和NAA 0.3 mg L^-1；叶培养的最适通用培养基为MS附加6-BA 3 mg L^-1和NAA 0.3 mg L^-1。

Abstract:

The explants from seed and leaf segments of Delphinium grandiflorum were used to establish culture in vitro and regeneration system. The experiments showed that most explants induced calli, few of them could form plantlets directly from seed or leaf segments. Under suitable culture condition, high regeneration rates could be obtained from explants of seed and leaf segments, each explant regenerating 10-20 plantlets. Optimum medium for seed segments of Delphinium grandiflorum was the modified MS medium (half of macronutrients and sucrose，10 times the amount of Vit B₁) supplemented with 3 mg L^-1 ZT and 0.3 mg L^-1 NAA，and for leaf segments，the medium of MS with 3 mg L^-1 6-BA and O.3 mg L^-1 NAA．