深山含笑的组织培养和快速繁殖

TISSUE CULTURE AND RAPID PROPAGATION OF MICHELLA MAUDLAE

摘要:

组织培养深山含笑的实验结果表明:利用休眠芽和种子萌发时实生苗的上胚轴及下胚轴为外植体均能诱导出愈伤组织和不定芽,其中无菌实生苗的上胚轴最易诱导出不定芽,无菌实生苗的下胚轴最易诱导出愈伤组织。外植体在MS+1.0-2.0 mgL^-1 2,4-D培养基上只产生愈伤组织；在MS+3.0 mg L^-1 BA+0.2 mg L^-1 NAA培养基上产生较多的不定芽和较多的愈伤组织；在MS+2.0 mg L^-1 2,4-D+3.0 mg L^-1 BA+0.2 mg L^-1 NAA培养基上易产生愈伤组织和大量的不定芽.愈伤组织在MS+2.0 mg L^-1 BA+0.2 mg L^-1 NAA培养基上能产生大量的不定芽,不定芽在此培养基上增殖速度快,增殖速度可达6.30倍.试管内小苗在1/2MS+0.5 mg L^-1 NAA培养基上生根壮苗效果较好。试管苗移栽成活率为92%。

Abstract:

Explants from dormant bud segments and from epicotyls and hypocotyl of the seedlings of Michelia maudiae Dunn were cultured on MS medium supplemented with various concentrations of NAA, 2,4-D, BA alone or in combination. Medium with 2,4-D could only produce calluses. A large number of adventitious buds and some calluses were observed on medium containing BA (3.0 mg L^-1) and NAA (0.2 mg L^-1). Calluses from original explants generated a maximum number of shoots in subculture on MS medium supplemented with BA (2.0 mg L^-1)and NAA (0.2 tag L^-1)．Planflets rooted well on 1/2 Ms medium containing 0.5 mg L NAA with 1.0％ active carbon． The plantlets were transferred to pots containing a perlite/coconut fibre and (2：2：1) mixture which grew normally with a survival rate of 92％．