干旱、盐和低温胁迫对水稻幼苗脯氨酸含量的影响

EFFECTS OF DROUGHT, SALT AND CHILLING STRESSES ON PROLINE ACCUMULATION IN SHOOT OF RICE SEEDLINGS

摘要:

以三叶期的水稻幼苗为材料,研究了干旱(-0.6 MPa PEG模拟)、盐(0.15 mol/L NaCl)和低温(6℃)胁迫下,不同水稻品种脯氨酸积累的变化。结果表明,干旱、盐和低温胁迫下稻苗均可积累脯氨酸,且随着胁迫时间的延长而加剧。在同一胁迫条件下,耐性强的品种脯氨酸积累较少,而敏感品种脯氨酸积累则较多。脯氨酸的积累不宜作为稻苗抗逆性的筛选指标。

Abstract:

Drought, salt and chilling effects on proline accumulation were studied in 3-leaf-stage seedlings of three rice (Oryza sativa L.) varieties: IAC (sensitive to drought and salt, and tolerant to low temperature), Dahuanggu (salt-resistant but sensitive to low temperature) and Guixi (drought-tolerant). Treatments were carried out at -0.6 MPa PEG (simulation) for drought, 0.15 mol/L NaC1 for salinity, and 6 ℃ for chilling stresses. Proline content in aboveground part of rice seedlings were tested. The results showed that proline accumulation in rice seedlings of various varieties was seen under all stresses，and the proline content increased with increasing time of stress．Sensitive variety accumulated more proline content than tolerant one. It seems，however, that proline accumulation can not be used as a screening index of resistance to stres ses in rice seedlings.