AgNO3对大白菜子叶芽再生的促进作用

STIMULATION EFFECT OF SILVER NITRATE ON SHOOT REGENERATION IN COTYLEDON TISSUE CULTURE OF BRASSIA CAMPESTRIS

摘要:

以大白菜"O2号杂交早皇白"无菌苗的子叶为材料,用附加BA 2.O mg L^-1、NAA 0.1-1.0 mg L^-1的MS培养基培养,能直接诱导分化出芽.最适激素比例为BA 2.O mg L^-1、NAA 0.5 mg L^-1,芽的分化率为31.6%。在上述培养基里添加2 mg L^-1的AgNO₃能使芽的再生频率提高至86.5%。

Abstract:

A tissue culture system for obtaining high frequency shoot regeneration of Brassica campestris was studied. Shoots could be induced directly from cotyledons cultured on MS medium supplemented with 2.0 mg L^-1 BA and 0.5 mg L^-1 NAA, the shoot regeneration rate being 31.6%. Addition of 2.0 mg L^-1 AgNO₃ in the medium could greatly increase the frequency of shoot regeneration, and the shoot regeneration rate reached to 86.5%.