以大白菜"O2号杂交早皇白"无菌苗的子叶为材料,用附加BA 2.O mg L-1、NAA 0.1-1.0 mg L-1的MS培养基培养,能直接诱导分化出芽.最适激素比例为BA 2.O mg L-1、NAA 0.5 mg L-1,芽的分化率为31.6%。在上述培养基里添加2 mg L-1的AgNO3能使芽的再生频率提高至86.5%。
A tissue culture system for obtaining high frequency shoot regeneration of Brassica campestris was studied. Shoots could be induced directly from cotyledons cultured on MS medium supplemented with 2.0 mg L-1 BA and 0.5 mg L-1 NAA, the shoot regeneration rate being 31.6%. Addition of 2.0 mg L-1 AgNO3 in the medium could greatly increase the frequency of shoot regeneration, and the shoot regeneration rate reached to 86.5%.
杜虹,庄东红,黄文华. AgNO3对大白菜子叶芽再生的促进作用[J].热带亚热带植物学报,2000,8(2):109~112
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