香根草不同处植体诿导体细胞胚胎发生和器官发生

SOMATIC EMBRYOGENESIS AND SHOOT FORMATION FROM EXPLANTS OF VETIVERIA ZIZANIOIDES

摘要:

以禾本科植物香根草(Vetiveria zizanioides)的嫩叶鞘基部切段为外植体,再取其试管苗的基部切段诱导体细胞胚胎发生和芽的器官发生。结果表明生长素是诱导外植体脱分化和形成体细胞胚的关键因子,而芽的形成则源于体细胞胚的萌发,它们是由NAA或低浓度的2,4-D所诱导的。通过研究建立了有效的香根草循环诱导再生体系和试管苗繁殖体系,为进一步研究香根草的生物技术奠定了良好的基础。

Abstract:

The explants of young sheath and in vitro basal shoot fragments of vetiver (Vetiveria zizanioides) were used to study the somatic embryogenesis and shoot formation. The results showed that auxin was the key factor for the induction of somatic embryogenesis from the explants. Shoot organogenesis originated from the germination of somatic embryos which could be induced on induction media supplemented with NAA or low concentration of 2,4-D. An efficient recycle induction, regeneration and propagation system of vetiver were established.