Ri T-DNA转化对黄瓜毛状根内源激素水平的影响

EFFECT OF Ri T-DNA TRANSFORMATION ON THE LEVELS OF ENDOGENOUS PHYTOHORMONES IN HAIRY ROOTS OF CUCUMIS SATIVUS L.

摘要:

利用酶联免疫法和气相色谱法分别测定发根农杆菌R1000转化黄瓜子叶产生的三种毛状根内源IAA、ABA、iPAs、ZRs、GA1+3、GA4+7和乙烯含量的变化。结果表明:内源IAA、ABA和GA1+3含量都以III型毛状根最高,II型毛状根次之,I型毛状根最低；但GA4+7含量顺序恰好与之相反,III型毛状根GA4+7含量低至检测不出。黄瓜对照根和毛状根的GA1+3和ipAs含量都分别比GA4+7和ZRs高得多,但毛状根的ipAS和ZRs含量均高于对照根。发根农杆菌R1000菌株转化黄瓜子叶产生的II型毛状根和III型毛状根产生乙烯的能力比对照根强得多。

Abstract:

The contents of endogenous IAA, ABA, iPAs, ZRs, GA1+3, GA4+7 and ethylene in three phenotypes of cucumber hairy roots transformed by Ri T-DNA from Agrobacterium rhizogenes R1000 were assayed by enzyme-linked immunosorbent assay (ELISA) and gas chromatography, respectively. The 3 phenotypes of cucumber hairy roots were described by the authors and published in Acta Botanica Sinica 1998, 40(5).470-473. The results showed that the contents of endogenous IAA, ABA and GAl +3 in hairy roots were highest in phenotype III，and less in descending order in phenotype II and phenotype I，whereas the amount of GA4+ 7 was in oppsite order，GA4+ 7 content in hairy roots of phenotype III was lowest and could not be detected．The contents of iPAs and GAl+3 in control roots and RlO00-transformed hairy roots were much higher than those of ZRs and GA4+ 7，respectively．However，the contents of iPAs and ZRs in R1000-transformed hairy roots were higher than those in control roots．The ability of ethylene production in R1000-transformed hairy roots was much higher than that in cucumber control roots．