利用RAPD技术快速鉴定番茄体细胞无性系变异

RAPID IDENTIFICATION OF TOMATO SOMACLONAL VARIATION WITH RAPD

摘要:

从以镰刀菌酸为选择剂筛选的番茄再生植株和未经筛选的植株叶片中提取DNA，建立了适合番茄RAPD分析的PCR条件，进一步在60个随机引物中找到了4个可用于鉴定四个番茄品种体细胞无性系变异的引物。利用该法鉴定体细胞无性系变异不仅简单、迅速、可靠，而且因DNA的用量少，不影响被检植株的后期生长，可尽早淘汰那些生理适应性而非分子水平发生变异的再生植株。

Abstract:

Total DNA was extracted from the leaves of generated tomato plants that werescreened and non-screened with fusaric acid, and was used to make the optimization forRAPD analysis. Four of the 60 random primers were adapted for the identification ofsomaclonal variation. This kind of method is not only simple, rapid, but also accurate.Furthermore, because the quantity of the DNA template is less, it is not influential in thegrowth of the tested plants. Therefore, this method can help us to pick out the plants that are physiologica11y tolerant， whereas mutant plants are reserved.