钙调素在IAA和6-BA诱导绿豆下胚轴原生质体膨大过程中的含量变化

CHANGES IN CONTENT OF CALMODULIN IN IAA-AND 6-BA-INDUCED SWELLING OF PROTOPLASTS FROM HYPOCOTYL IN ETIOLATED MUNG BEAN SEEDLINGS

摘要:

含钙培养液和含激素培养液中的绿豆下胚轴原生质体在培养30min时分别检测到钙调素（CaM）峰，在含钙培养液中加入IAA或6-8ACaM含量急剧降低，这与先前的试验结果即⁴⁵Ca²⁺积累增多和体积膨大正好对应。异博定（verapamil）、LaCl₃、EGTA或W7可使激素+CaCl₂处理的CaM含量不同程度地回升，甚至接近或超过对照的水平。而A23187处理或K⁺、Zn²⁺等代替Ca²⁺则使CaM含量保持在与激素处理相似的低水平。这也与先前观察到的⁴⁵Ca²⁺积累和体积的变化相对应。表明钙调素在IAA和6-8A诱导绿豆下胚轴原生质体膨大过程中的境与Ca²⁺共同起着调节作用。

Abstract:

Calmodulin (CaM) content in protoplasts cultured with CaCl₂-or IAA (or 6-BA)-bearing medium raised after being incubated for 30 minutes. When treated with IAA (or6-BA)+CaCl₂, the CaM content decreased sharply, which just coincided with the increase of ⁴⁵Ca²⁺ accumulation and protoplast swelling as indicated in our previous studies. Eitherverapadril, LaCl₃, EGTA or W7 could increase the low CaM content to an approximate oreven higher value than that of control. A23187 and cations such as K⁺ and Zn²⁺,however, could reduce CaM level just as the influences of IAA or 6-BA with Ca²⁺, It was also consistent with the changes of ⁴⁵Ca²⁺ accumulation and protoplast volume．These results showed that CaM did act with Ca²⁺ during the modulation of protoplast swelling induced by IAA or 6-BA．