中国地胆草属和假地胆草属(菊科)分合的分子依据

MOLECULAR EVIDENCES ON SEPARATION AND COMBINATION OF THE GENERA ELEPHANTOPUS L. AND PSEUDELEPHANTOPUS ROHR (COMPOSITAE) IN CHINA

摘要:

为探讨中国菊科地胆革属和假地胆草属在DNA分子水平上的亲缘关系以及这两个属的分合问题，以香港野生的地胆草属植物地胆草（ElephantopusscaberL）、白花地胆草（E.mollisHK．B）和香港、台湾归化的假地胆草属植物假地胆草（Pseudelephantopusspicatus（BJussexAublet）CF.Baker）为材料，选取6种18－24mer和5种10mer的随机单引物进行任意引物PCR（AN－PCR）和随机扩增多态DNA（RAP）分析，以相似没指数值作亲缘关系分析。初步结果显示：2种地胆草与1种假地胆草之间的DNA指纹图谱差异较大，提示地肥草属与假地胆草属植物的亲缘关系较远，属于属间差异。这与形态学、组织学和细胞学结果呈相关性，认为这两个属合并成一个广义的地胆草属是不合适的。

Abstract:

Two Elephantopus species and one Pseudelephantopus species have been placed inone single genus Elephantopus L. sensu lato by some earlier systematists. The genomicprofile obtained by amplifying the DNA with different single primers were distinctive tothese three species and two genera. The Similarity Indexes indicated that Elephantopus mollisis more closely related to E. scaber than to Pseudelephantopus spicatus. The present studyindicated that the Elephantopus scaber L., and its closed species E. mollis as well as Pseudetephantopus spicatus were genetically to be distinguished． The estimates of genomic DNA fingerprints based on Similarity Index values ca lculated from amplified DNA band profiles show a dose association with known levds of morphological and histological， cytological relatedness．