花生体细胞胚的诱导及其植株再生

SOMATIC EMBRYOGENESIS AND PLANT REGENERATION FROM AXES OF PEANUT EMBRYOS

摘要:

采用不同成熟度的花生胚轴为外植体进行体细胞胚诱导及植株再生研究，结果表明，成熟胚轴在高浓度2，4－D的MS培养基中，经过30d左右的培养，可直接诱导产生出大量的体细胞胚，含40 mg L^-1 2，4－D的培养基中体细胞胚的诱导率达100％，平均每个外植体产生11。58个体细胞胚。体细胞胚的继代培养需降低2，4－D的浓度（1－20 mg L^-1）。未成熟胚轴的体细胞胚诱导及继代培养的2，4－D浓度宜为10 mg L^-1。将诱导的体细胞胚转接到合5－10 mg L^-1 BA的MS培养基中，体细胞胚能够萌发再生成无根小植株，将其转接到生根培养基中可获得完整小植株。

Abstract:

Somatic embryos could be induced from axes of mature peanut embryos culturedon hasal MS medium containing high concentration of 2,4-D (40 mg L^-1) with 0.5 mg L^-1 kinetin, 30% sucrose and 0.9% agar for 30 days, the induction rate being 100 percent,and the mean embryo number per explant reaching 11.58. Lower concentrations of 2,4-D(10-20 mg L^-1) were suitable for somatic embroid differentiation in subculture. However,10 mg L^-1 2,4-D was the optimum concentration for inducing somatic embryos both in primary culture and in subculture by immature embryo axes．Somatic embryos could easily germinate on MS medium with 5-10 mg L^-1 BA. The plantiets were obtained after the shoots were tranferred to root-inducing medium, MS+ 2 mg L^-1 NAA．